Interlaboratory evaluation of hematocytometer method of inoculum preparation for testing antifungal susceptibilities of filamentous fungi.
نویسندگان
چکیده
A three-laboratory study was performed to evaluate conidium counting in a hematocytometer as a technique of inoculum preparation for susceptibility testing of Aspergillus spp. In addition, inocula were quantified by colony counting and optical density determination. The agreement and correlation coefficient between conidium and colony quantifications were 89.2% and 0.73 (P < 0.01). Correlations with optical density determination were not significant.
منابع مشابه
Inoculum standardization for antifungal susceptibility testing of filamentous fungi pathogenic for humans.
Two methods of inoculum preparation for filamentous fungi were compared: counting with a hematocytometer and spectrophotometric adjustment. One hundred eighty-two filamentous fungi pathogenic for humans were used. Colony counts were done for all inoculum preparations. The agreement between the hematocytometer counts and the colony counts (CFU per milliliter) was 97.2%. The reproducibility betwe...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 41 11 شماره
صفحات -
تاریخ انتشار 2003